Name: Lysozyme powder
Brand: Joyous Pellets Supplement raw materials, cosmetic ingredients, weight
Availability: InStock
Rating: 5 (1 reviews)

Lysozyme, also known as muramidase or N-acetylmuramide glycanohydrlase, is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. According to their different sources, lysozyme is divided into plant lysozyme, animal lysozyme, microbial lysozyme and egg white lysozyme. Joyous is lysozyme hydrochloride derived from egg whites

Product Name

lysozyme

Specification

99%，20,000 iu/g

CAS No

9066-59-5

Appearance

off white powder

EINECS

2329540

Storage conditions

Refrigerated at -20℃. Incompatible with strong oxidizing agents

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Desciption

Product information
What is Lysozyme powder?
Lysozyme Hydrochloride is a hydrochloride of a basic polypeptide obtained from albumen of hen's egg, and has an activity to hydrolyze mucopolysaccha-rides.It contains not less than 0.9 mg (potency) of lysozyme per mg, calculated on the dried basis.Lysozyme Hydrochloride occurs as white,crystals, or crystalline or amorphous powder.It is freely soluble in water, and practically insoluble in ethanol (99.5).It is hygroscopic.The pH of a solution of Lysozyme Hydrochloride (3 in 200) is between 3.0 and 5.0.Optimum reaction temperature and pH: 35℃, pH6.2
Joyous Lysozyme powder specification

Terms	Standard	Terms	Standard
Product Name	Lysozyme	Appearance	Off white powder
Assay	98%	Loss on Drying	≤2%
Total Heavy Metals	≤10 ppm	Ash	≤2%
As	≤0.5ppm	Total Plate Count	≤750 cfu/g
Pb	≤0.5ppm	Yeast and Mold	≤100 cfu/g
Cd	≤0.5ppm	Coliforms	Negative
Hg	≤0.1ppm	E.Coli	Negative
Packing	Pack in 25kgs paper-drums, inner by double plastic bag	Shelf life	24 months under the above condition, and in its original package

Antibacterial mechanism and preparation method of Lysozyme
Lysozyme can effectively hydrolyze the peptidoglycan of the bacterial cell wall. Its hydrolysis site is the β-carbon atom between the 1st carbon atom of N-acetylmuramic acid (NAM) and the 4th carbon atom of N-acetylglucosamine (NAG). 1.4 Glycosidic bond. Peptidoglycan is the main component of the bacterial cell wall. It is composed of NAM, NAG and a peptide "tail" (composed of 4 amino acid residues). NAM and NAG are connected through β-1.4 glycosidic bonds, and the peptide "tail" is connected through D -The lactyl carboxyl group is connected to the 3rd carbon atom of NAM, and the peptide tails are connected through peptide "bridges" (peptide bonds or a few amino acids). NAM, NAG, peptide "tails" and peptide "bridges" are composed of The multi-layered network structure of peptidoglycan serves as the skeleton of the cell wall. Breaking of any chemical bonds in the above structure can cause damage to the bacterial cell wall. For Gram-positive bacteria (G+), such as Micrococcus luteus, Bacillus subtilis, or Micrococcus lyticum, and Gram-negative bacteria (G-), such as Escherichia coli, Proteus, Shigella dysenteriae, Pneumoniae, etc., The content of peptidoglycan in their cell walls is different. The cell wall of G+ bacteria is almost entirely composed of peptidoglycan, while only the inner wall layer of G- bacteria is peptidoglycan. Therefore, lysozyme is stronger at destroying the cell wall of G+ bacteria than G- bacteria.

Preparation
At present, lysozyme can be extracted from egg white and eggshell membrane. Commonly used methods include affinity chromatography, ion exchange resin method, direct crystallization method and polyacrylic acid precipitation method.
affinity chromatography
Affinity chromatography is a chromatography technology designed to take advantage of the biological specificity of proteins and enzymes, that is, the specific affinity between proteins or enzymes and their ligands. After the enzyme-substrate complex is formed, the complex can be separated under certain conditions to obtain the pure enzyme. Commonly used adsorbents are chitin and its derivatives, such as: chitin powder, carboxymethyl chitin, chitin-embedded cellulose, deaminated chitin powder, N-acylated chitosan , Deamination regeneration of chitin gel.
Ion exchange resin method
The ion exchange method uses the exchange reaction between the ion exchange agent and the ions in the solution for separation. The separation effect is good and it is widely used for the enrichment of trace components. The general process is: fresh eggs - pretreatment - stirring adsorption - column elution - isoelectric precipitation - dialysis - spray drying - finished product.
Lysozyme has a positive charge in a wide range of pH values below the isoelectric point and can be adsorbed on weakly acidic cation exchange resins. After elution, it salts out to precipitate lysozyme, which can then be refined to obtain finished products. Egg white adsorbs 724 resin, elutes with washing buffer, salts out and dialyzes with ammonium sulfate solution, uses NaOH to remove alkaline proteins, freeze-dries lysozyme, and lyophilized powder is precipitated and dried to obtain lysozyme. At 5-10°C, add 540kg of fresh egg white to 80kg of treated 724 resin, stir and adsorb for 6 hours, and let it stand overnight at 0-5°C. Pour out the upper layer of egg white, centrifuge the resin to dryness, repeatedly wash away the adhered egg protein with distilled water, then load the resin into the column, wash the resin with about 150L of 0.15mol/L, pH=6.5 phosphate buffer solution, and then use about 600L of Elute with 10% ammonium sulfate solution and collect the eluent. Add ammonium sulfate to the eluent so that the final ammonium sulfate content is 40%. A white precipitate will form. Leave it in a cold place overnight. Siphon the supernatant, filter and drain the precipitate, then dissolve the precipitate into a thin paste with 1x distilled water, then put it into a dialysis bag, and dialyze the distilled water for about 24 hours at about 5°C, changing the water 2-3 times in the middle. . Centrifuge to remove the precipitate, wash the precipitate once with a small amount of water, and combine the washing liquid with the centrifugal liquid. Slowly add 1mol/L sodium hydroxide solution to the dialysate and stir continuously to raise the pH value to 8.0-9.0. If there is white precipitate, remove it by centrifugation. Then use 3mol/L hydrochloric acid to adjust pH=5.0 and freeze-dry to obtain white flake lysozyme. You can also adjust the pH of the centrifuged liquid to 3.5 with 3mol/L hydrochloric acid, slowly add 5% solid sodium chloride while stirring, let it stand for 48 hours at a temperature of about 5°C, centrifuge, and wash the precipitate with 0°C acetone and dry. That is, lysozyme is obtained.
Salt direct crystallization method
Add a certain amount of salts such as iodide or carbonate to the egg white and adjust the pH to 9.5-10.0. Lysozyme will slowly precipitate in the form of crystals, while most of the protein is still in solution. After ultrafiltration, concentration and desalting, in order to obtain a purer product, crystallization was used to purify the enzyme solution, and after ultrafiltration treatment, the pH was adjusted to 9.5 with NaOH and centrifuged to remove it. Add NaCl to the supernatant to 5% with gentle stirring, and let it stand for a week to obtain crude crystals. The crystals are dissolved in acetic acid water with a pH of 4.6. After the insoluble matter is separated, the crystals are recrystallized. The final yield of the crystals is about 60%. That is, nearly 1.3 g of recrystallized product can be obtained per kilogram of egg white, and the activity is measured at 8000U/mg.
Polyacrylic acid precipitation method
The polyacrylic acid precipitation method involves extracting and filtering the enzyme-containing eluate. First, it goes through an adsorption step. That is, the filtered clear solution is adjusted to pH 6.0 with 20% NaOH solution, and is stirred continuously during the pH adjustment process. A small amount of milky white precipitate precipitated, then add 15% polyacrylic acid, and white precipitate immediately precipitated. Add until the pH value is 3.0, let it stand for 17 hours for static analysis, and obtain a milky white gel that adheres to the bottom. In the second step of dissociation, the lysozyme polyacrylic acid condensate obtained in the previous step is dissociated. Add a small amount of distilled water and use 0.5 mol/L sodium carbonate to dissolve it. After transfer, adjust the pH value to 9.5. Add 5% CaCl2 solution to dissociate lysozyme polyacrylic acid until no precipitation occurs, and then filter to obtain a clear solution. In the third step of salting out, add 5% NaCI solution to the obtained clear solution and stir evenly. Place in the refrigerator and adjust the temperature to 0°C. After crystals precipitate, wash several times with absolute ethanol, dry and weigh in a constant temperature incubator at 40°C.

How is lysozyme stored?
It is recommended to store in a cool, dry environment away from light, storage temperature: below zero.
If stored for too long or under unfavorable storage conditions, the enzyme activity will be reduced to varying degrees; if the temperature and humidity are too high, the dosage needs to be appropriately increased during use.
Package and delivery

Delivery
1-80kg	80-300kg	More than 300kg
More suitable for express transportation, door-to-door	More suitable for air transportation,airport-to-airport	More suitable for ocean transportation,port-to-port
7-10 days for delivery time	3-4 days for delivery time	20-40 days for delivery time

What service we can provide
Joyous Health provides Lysozyme powder OEM services. We can customize formulas for customers, private labels, capsules, tablets, soft capsules and sachets, low MOQ, which is suitable for direct sales by brands. Our OEM product shipping channels are stable and delivery times are fast and worthy of customers' trust, we have served more than 300 brands.

Joyous Health Factory information and certifications
Xi'an Joyous Health Biotechnology Co., Ltd. was established in 2011. Over the past 12 years, Joyous Health has obtained ISO 9001, FDA, ISO 22000, and GMP certifications.
Joyous Health has an advanced laboratory to control quality, so that each batch of products undergoes strict testing, and has professional R&D personnel to provide customized product services.
For more details please click here

Where to buy Lysozyme powder?
We offer the best Lysozyme powder at the most competitive price.Lysozyme Powder bulk and wholesale powder have been 3rd lab-tested and verified for both product purity and identity.
We also provide Lysozyme powder in bulk order or wholesale according to client needs. Just submit the requirements in the bottom form, we will reply soon!

Contacts: Faith Feng
Email:faith@joyouspellets.com
Tel and Whatsapp:+86 17391747738

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Contact: Xi'an international enterprise center B building, Fengcheng 4 road, Xi'an City, Shaanxi province, China; info@joyouspellets.com; +86 17391747738

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